Multi-Scale Characterization of CRISPR Editing Outcomes Using rhAmpSeq™ and Tapestri® Single-Cell Multiomics

Nechama Kalter and the team from Professor Ayal Hendel's lab (Bar-Ilan University) characterized CRISPR genome editing outcomes in primary human T cells. Their work utilizes a novel two-tiered analytical workflow that paired the Integrated DNA Technologies’ (IDT) rhAmpSeq™ System for population-level confirmation with Mission Bio's Single-Cell Multiomics Platform, Tapestri®, for deep per-cell and per-allele confirmation. Ultimately, this paired workflow enables comprehensive characterization of CRISPR editing outcomes, from structural variations to cellular phenotypes, delivering the deep insights needed to advance a variety of applications including cell and gene therapy development research.

Targeting PDCD1, TCRA, and TCRB on-target sites alongside GUIDE-seq-nominated off-target loci, IDT's rhAmpSeq System established bulk baseline editing frequencies before Mission Bio's Tapestri platform resolved complex single-cell dynamics. This combined workflow achieved 99.77% and 99.93% indel detection sensitivity, identification of novel translocations in as few as 1 in 25,000 cells, and the ability to track editing-induced structural variations across a 12-day longitudinal time course. Download to see the data the team generated and learn how you can also use the rhAmpSeq and Tapestri workflow.